Back to Search Start Over

Discovery of Components Acting as the Obstacles in the Detection of Enteric Viruses from Berries.

Authors :
Zhao MY
Li D
Source :
Food and environmental virology [Food Environ Virol] 2020 Jun; Vol. 12 (2), pp. 191-197. Date of Electronic Publication: 2020 Apr 22.
Publication Year :
2020

Abstract

This study investigated the obstacles in detecting enteric viruses from berry fruits, which are on the one hand often associated with outbreaks of viral enteric disease, and on the other hand recognized as a challenging food matrix for molecular detection of enteric viruses. According to the ISO 15216 protocol, for soft fruit samples, virus extraction is by elution with agitation followed by precipitation with polyethylene glycol/NaCl. As a result, first, the phenolic content in the berry eluate was found to be weakly correlated with the detection of coliphage MS2 spiked in the berry samples. Second and more importantly, it was observed that the gel-like pellets formed after precipitation could entrap considerable portions of viruses from being further purified and recovered for detection, suggesting that the low virus detection sensitivity from berries is largely due to the pectin content with complicated chemical structures in the berry fruits. Future research is needed to solve this problem in a targeted way.

Details

Language :
English
ISSN :
1867-0342
Volume :
12
Issue :
2
Database :
MEDLINE
Journal :
Food and environmental virology
Publication Type :
Academic Journal
Accession number :
32323159
Full Text :
https://doi.org/10.1007/s12560-020-09428-y