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Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages.
- Source :
-
Acta tropica [Acta Trop] 2020 Jul; Vol. 207, pp. 105502. Date of Electronic Publication: 2020 Apr 19. - Publication Year :
- 2020
-
Abstract
- Malaria is transmitted by Plasmodium parasites through the bite of female Anopheles mosquitoes. One of the most important mosquito vectors in the Greater Mekong Subregion is Anopheles dirus. This study reports RNA sequencing (RNA-Seq) transcriptome analysis of An. dirus at 18 hours and 7 days after a P. vivax-infected blood meal, which represent infection at the ookinete and oocyst parasite developmental stages, respectively. Following infection, 582 An. dirus transcripts were modulated. The 2,408 P. vivax transcripts could be classified into ookinete-specific, two-stage, and oocyst-specific groups. Results were validated by quantitative reverse transcription polymerase chain reaction. Gene ontology analysis of the vector and parasite revealed several biological pathways for both, providing a better understanding of Anopheles-Plasmodium interactions at the ookinete and oocyst stages.<br />Competing Interests: Declaration of Competing Interest All authors declared no conflict of interest.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1873-6254
- Volume :
- 207
- Database :
- MEDLINE
- Journal :
- Acta tropica
- Publication Type :
- Academic Journal
- Accession number :
- 32320680
- Full Text :
- https://doi.org/10.1016/j.actatropica.2020.105502