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Depletion of resident muscle stem cells negatively impacts running volume, physical function, and muscle fiber hypertrophy in response to lifelong physical activity.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2020 Jun 01; Vol. 318 (6), pp. C1178-C1188. Date of Electronic Publication: 2020 Apr 22. - Publication Year :
- 2020
-
Abstract
- To date, studies that have aimed to investigate the role of satellite cells during adult skeletal muscle adaptation and hypertrophy have utilized a nontranslational stimulus and/or have been performed over a relatively short time frame. Although it has been shown that satellite cell depletion throughout adulthood does not drive skeletal muscle loss in sedentary mice, it remains unknown how satellite cells participate in skeletal muscle adaptation to long-term physical activity. The current study was designed to determine whether reduced satellite cell content throughout adulthood would influence the transcriptome-wide response to physical activity and diminish the adaptive response of skeletal muscle. We administered vehicle or tamoxifen to adult Pax7-diphtheria toxin A (DTA) mice to deplete satellite cells and assigned them to sedentary or wheel-running conditions for 13 mo. Satellite cell depletion throughout adulthood reduced balance and coordination, overall running volume, and the size of muscle proprioceptors (spindle fibers). Furthermore, satellite cell participation was necessary for optimal muscle fiber hypertrophy but not adaptations in fiber type distribution in response to lifelong physical activity. Transcriptome-wide analysis of the plantaris and soleus revealed that satellite cell function is muscle type specific; satellite cell-dependent myonuclear accretion was apparent in oxidative muscles, whereas initiation of G protein-coupled receptor (GPCR) signaling in the glycolytic plantaris may require satellite cells to induce optimal adaptations to long-term physical activity. These findings suggest that satellite cells play a role in preserving physical function during aging and influence muscle adaptation during sustained periods of physical activity.
- Subjects :
- Adaptation, Physiological
Animals
Diphtheria Toxin genetics
Female
Gene Expression Regulation
Glycolysis
Hypertrophy
Mice, Transgenic
Muscle Fibers, Skeletal metabolism
Oxidation-Reduction
PAX7 Transcription Factor genetics
Peptide Fragments genetics
RNA, Untranslated genetics
Satellite Cells, Skeletal Muscle metabolism
Time Factors
Muscle Fibers, Skeletal pathology
Physical Conditioning, Animal
Running
Satellite Cells, Skeletal Muscle pathology
Sedentary Behavior
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1563
- Volume :
- 318
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 32320286
- Full Text :
- https://doi.org/10.1152/ajpcell.00090.2020