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Paeonol protects against testicular ischaemia-reperfusion injury in rats through inhibition of oxidative stress and inflammation.
- Source :
-
Andrologia [Andrologia] 2020 Jul; Vol. 52 (6), pp. e13599. Date of Electronic Publication: 2020 Apr 21. - Publication Year :
- 2020
-
Abstract
- Ischaemia-reperfusion (IR) is the most common form of testicular injury that results in oxidative damage and inflammation ending by subinfertility. Paeonol, a natural phenolic compound, exhibits antioxidant and anti-inflammatory effects. Thus, the present study investigated the role of paeonol in rat testicular IR injury. Thirty adult Wistar rats were randomly divided into five groups; sham, sham treated with paeonol, IR injury, and IR pre-treated with paeonol at low and high doses. Serum testosterone and testicular levels of malondialdehyde and reduced glutathione (GSH) besides superoxide dismutase (SOD) activity were determined. Gene quantifications for tumour necrosis factor-α (TNF-α), hypoxia-inducible factor-1α (HIF-1α) and heat shock protein 70 (HSP70) were also assessed. Histopathological pictures and the immunohistochemical expression of testicular nuclear factor erythroid 2-related factor 2 (Nrf2), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were shown. Pre-treatment with paeonol prevented the drop in serum testosterone, alongside with improvement of testicular malondialdehyde and GSH levels plus SOD activity. Paeonol regained the normal spermatogenesis with prevention of IR-induced increase in TNF-α, HIF-1α and HSP70 gene expression besides IL-1β and IL-6 immunostaining and reduction in Nrf2 protein expression. Paeonol exerted a dose-dependent beneficial effect on testicular IR injury. This effect was achieved by its antioxidant and anti-inflammatory effects.<br /> (© 2020 Blackwell Verlag GmbH.)
- Subjects :
- Animals
Gene Expression drug effects
Glutathione drug effects
Glutathione metabolism
HSP70 Heat-Shock Proteins drug effects
HSP70 Heat-Shock Proteins genetics
HSP70 Heat-Shock Proteins metabolism
Hypoxia-Inducible Factor 1, alpha Subunit drug effects
Hypoxia-Inducible Factor 1, alpha Subunit genetics
Hypoxia-Inducible Factor 1, alpha Subunit metabolism
Immunohistochemistry
Inflammation metabolism
Inflammation pathology
Interleukin-1beta drug effects
Interleukin-1beta metabolism
Interleukin-6 metabolism
Male
Malondialdehyde metabolism
NF-E2-Related Factor 2 drug effects
NF-E2-Related Factor 2 metabolism
Rats
Reperfusion Injury pathology
Spermatic Cord Torsion pathology
Superoxide Dismutase drug effects
Superoxide Dismutase metabolism
Testis metabolism
Testis pathology
Testosterone metabolism
Tumor Necrosis Factor-alpha drug effects
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha metabolism
Acetophenones pharmacology
Oxidative Stress drug effects
Reperfusion Injury metabolism
Spermatic Cord Torsion metabolism
Testis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0272
- Volume :
- 52
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Andrologia
- Publication Type :
- Academic Journal
- Accession number :
- 32314822
- Full Text :
- https://doi.org/10.1111/and.13599