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Simple and Rapid Detection of Glycoforms by "Lectin Inhibition" Assay.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2132, pp. 165-171. - Publication Year :
- 2020
-
Abstract
- Glycoforms are otherwise identical proteins with different glycosylation. A lectin, Sambucus sieboldiana agglutinin (SSA), specifically binds glycoforms having α2,6-sialyl residues. The binding is found to inhibit antigen-antibody reaction; e.g., SSA inhibits anti-transferrin antibody binding to α2,6-sialylated transferrin (Tf) (SSA inhibition). SSA inhibition is not observed with other Tf glycoforms, indicating that the inhibition is glycoform-specific. Here we describe the application of SSA inhibition to ELISA as a specific assay for quantifying α2,6-sialylated Tf.
- Subjects :
- Antibodies metabolism
Antigen-Antibody Reactions drug effects
Blood Chemical Analysis
Enzyme-Linked Immunosorbent Assay
Glycosylation
Humans
N-Acetylneuraminic Acid metabolism
Transferrin immunology
Plant Lectins pharmacology
Ribosome Inactivating Proteins pharmacology
Sambucus metabolism
Transferrin analysis
Transferrin chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2132
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32306325
- Full Text :
- https://doi.org/10.1007/978-1-0716-0430-4_17