The Ca 2+ -Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation.

Authors :: Krasitskaya VV
Goncharova NS
Biriukov VV
Bashmakova EE
Kabilov MR
Baykov IK
Sokolov AE
Frank LA
Source :: Photochemistry and photobiology [Photochem Photobiol] 2020 Sep; Vol. 96 (5), pp. 1041-1046. Date of Electronic Publication: 2020 May 28.
Publication Year :: 2020
Abstract: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca <superscript>2+</superscript> -regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.<br /> (© 2020 American Society for Photobiology.)

Subjects :: Aptamers, Nucleotide chemistry
Base Sequence
Biomarkers analysis
Limit of Detection
Luminescent Measurements
Calcium chemistry
Luminescent Proteins chemistry
SELEX Aptamer Technique methods
Troponin I analysis

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