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Identifying the hub gene in gastric cancer by bioinformatics analysis and in vitro experiments.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2020 Jun; Vol. 19 (11), pp. 1326-1337. Date of Electronic Publication: 2020 Apr 15. - Publication Year :
- 2020
-
Abstract
- Gastric cancer (GC) is one of the main causes of the high death rate in the world. But the molecular mechanisms of GC carcinogenesis remain little known. This study aimed to identify novel promising biomarkers of GC and reveal its potential molecular mechanisms by integrating bioinformatics analysis. We screened the overlapped differentially expressed genes (DEGs) of TCGA and several GEO datasets. Among these DEGs, we used protein-protein interactions network analysis to recognize the hub genes. Moreover, functional enrichment analysis including GO and KEGG pathway analysis and gene set enrichment analysis (GSEA) were performed to study the role of DEGs and potential underlying mechanisms of GC. Based on integrated bioinformatics analysis, SERPINH1, COL1A2, COL8A1, COL4A1, COL5A1, COL12A1, and COL1A1 were screened as candidate diagnostic marker genes. In addition, SERPINH1 was identified as a core gene in the regulation of GC development. Furthermore, we confirmed that SERPINH1 could promote the proliferation, migration, and cell cycle of GC cells. Our present study demonstrated that SERPINH1 was a core therapeutic biomarker in the regulation of candidate genes involved in GC progression.
- Subjects :
- Apoptosis genetics
Cell Cycle genetics
Cell Line, Tumor
Cell Movement genetics
Cell Proliferation genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Ontology
HSP47 Heat-Shock Proteins genetics
HSP47 Heat-Shock Proteins metabolism
Humans
Protein Interaction Mapping
Tumor Stem Cell Assay
Up-Regulation genetics
Computational Biology
Gene Regulatory Networks
Stomach Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 19
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 32293980
- Full Text :
- https://doi.org/10.1080/15384101.2020.1749789