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Immunomodulatory role of Interleukin-33 in large vessel vasculitis.
- Source :
-
Scientific reports [Sci Rep] 2020 Apr 14; Vol. 10 (1), pp. 6405. Date of Electronic Publication: 2020 Apr 14. - Publication Year :
- 2020
-
Abstract
- The mechanisms regulating inflammation in large vessels vasculitis (LVV) are poorly understood. Interleukin 33 (IL-33) has been shown to license innate and adaptive immunity by enhancing Th2 cytokines production. We aimed to examine the role of IL-33 in the immunomodulation of T cell activation in LVV. T cell homeostasis and cytokines production were determined in peripheral blood from 52 patients with giant cell arteritis (GCA) and 50 healthy donors (HD), using Luminex assay, flow cytometry, quantitative RT-PCR and by immunofluorescence analysis in inflammatory aorta lesions. We found increased level of IL-33 and its receptor ST2/IL-1R4 in the serum of patient with LVV. Endothelial cells were the main source of IL-33, whereas Th2 cells, Tregs and mast cells (MC) express ST2 in LVV vessels. IL-33 had a direct immunomodulatory impact by increasing Th2 and Tregs. IL-33 and MC further enhanced Th2 and regulatory responses by inducing a 6.1 fold increased proportion of Tregs (p = 0.008). Stimulation of MC by IL-33 increased indoleamine 2 3-dioxygenase (IDO) activity and IL-2 secretion. IL-33 mRNA expression was significantly correlated with the expression of IL-10 and TGF-β within aorta inflammatory lesions. To conclude, our findings suggest that IL-33 may exert a critical immunoregulatory role in promoting Tregs and Th2 cells in LVV.
- Subjects :
- Aged
Aged, 80 and over
Female
Giant Cell Arteritis blood
Giant Cell Arteritis pathology
Humans
Inflammation blood
Inflammation immunology
Interleukin-1 Receptor-Like 1 Protein metabolism
Interleukin-33 blood
Male
Mast Cells immunology
Th2 Cells immunology
Giant Cell Arteritis immunology
Interleukin-33 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32286393
- Full Text :
- https://doi.org/10.1038/s41598-020-63042-3