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Incorporation of non-acetylated hexosamines into plasma membrane glycoproteins of liver cells after galactosamine injection.
- Source :
-
Biological chemistry Hoppe-Seyler [Biol Chem Hoppe Seyler] 1988 Sep; Vol. 369 (9), pp. 957-64. - Publication Year :
- 1988
-
Abstract
- The following procedure for the detection of non-acetylated amino sugars in the plasma membrane was established: i) derivatization of free amino groups with dansyl-chloride, ii) hydrolysis with 3 M HCl (for 4 h at 105 degrees C) to liberate the dansylated carbohydrate moieties from the plasma membrane, iii) purification of the dansylated amino sugars by paper chromatography and subsequent analysis by thin-layer chromatography. Using this procedure, plasma membranes from rat liver were analysed after injection of D-[14C]galactosamine. For this purpose, rats were divided into three groups: the first received D-galactosamine.HCl at a dose of 2 mg/kg b.w., the second at a dose of 75 mg/kg b.w. and the third at a hepatitis-inducing dose of 260 mg/kg b.w.. In all three groups the majority of the protein-bound radioactivity in the plasma membrane was not dansylated, thus representing N-acetylated amino sugars. At a dose of 2 mg/kg, only 0.34% of the protein-bound radioactivity in the plasma membrane reacted with dansyl-chloride. At a dose of 70 mg/kg this value increased to 1.9%. At 260 mg/kg the value was 3.6%. These results indicate that the incorporation of non-acetylated amino sugar into the plasma membrane was dose-dependent and reached 90 pmol per mg plasma membrane protein during galactosamine injury. However, this incorporation of non-acetylated amino sugars into the plasma membrane did not represent a pathological mechanism responsible for the onset of the galactosamine-induced liver injury.(ABSTRACT TRUNCATED AT 250 WORDS)
Details
- Language :
- English
- ISSN :
- 0177-3593
- Volume :
- 369
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biological chemistry Hoppe-Seyler
- Publication Type :
- Academic Journal
- Accession number :
- 3228492
- Full Text :
- https://doi.org/10.1515/bchm3.1988.369.2.957