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Chlorhexidine preserves the hybrid layer in vitro after 10-years aging.
- Source :
-
Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2020 May; Vol. 36 (5), pp. 672-680. Date of Electronic Publication: 2020 Apr 10. - Publication Year :
- 2020
-
Abstract
- Objective: The present study investigated the ability of a chlorhexidine (CHX)-containing primer (0.2% aqueous solution) to inhibit dentinal enzymes, preserve the hybrid layer (HL) and remain within the HL, after 10 years of aging in artificial saliva at 37°C.<br />Methods: Non-carious extracted molars were assigned to two groups, cut into slabs exposing middle/deep dentin, etched and bonded with Adper Scotchbond 1XT (SB1XT) with or without 0.2% CHX aqueous solution pretreatment. Composite build-ups were made, and the specimens were cut in 1-mm thick bonded sticks. In situ zymography was performed on freshly prepared specimens (T <subscript>0</subscript> ) and specimens aged for 10 years (T <subscript>10-yr</subscript> ) at 37°C in artificial saliva, to investigate endogenous gelatinolytic activity within the HL. At T <subscript>10-yr</subscript> , specimens were also decalcified and embedded in epoxy resin for TEM analysis. Micro-Raman spectroscopy was performed at T <subscript>0</subscript> and T <subscript>10-yr</subscript> to evaluate the chemical profiles in intertubular dentin and the HL.<br />Results: In situ zymography showed less pronounced enzymatic activity in the CHX-pretreated group (p<0.05) regardless of aging, maintaining a similar level of fluorescence at T <subscript>0</subscript> and T <subscript>10-yr</subscript> (p>0.05). TEM results showed that 98% of the HL had been degraded in the control group, while 95% of the HL was intact in the experimental group. Moreover, all the Raman spectra peaks assigned to CHX could be identified only in the CHX-pretreated group (T <subscript>0</subscript> and T <subscript>10-yr</subscript> ).<br />Significance: In vitro, CHX remains in the HL after 10 years with its inhibitory effect preserved. This may be the underlying factor for HL preservation after this long aging period.<br /> (Copyright © 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0097
- Volume :
- 36
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Dental materials : official publication of the Academy of Dental Materials
- Publication Type :
- Academic Journal
- Accession number :
- 32284197
- Full Text :
- https://doi.org/10.1016/j.dental.2020.03.009