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An amplification label of core-shell CdSe@CdS QD sensitized GO for a signal-on photoelectrochemical immunosensor for amyloid β-protein.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2019 Feb 21; Vol. 7 (7), pp. 1142-1148. Date of Electronic Publication: 2019 Jan 30. - Publication Year :
- 2019
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Abstract
- In this work, the heterostructures of Au nanoparticles loaded on 3D flower-like α-Fe <subscript>2</subscript> O <subscript>3</subscript> (Au NPs/α-Fe <subscript>2</subscript> O <subscript>3</subscript> ) as a platform and core-shell CdSe@CdS quantum dots sensitized graphene oxide (GO/CdSe@CdS QDs) as a signal amplification label were established in an ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor based on the "signal-on" type amplification method for detection of amyloid β-protein (Aβ). Specifically, the ultrahigh sensitivity of the proposed immunosensor came from two main properties. First, Au NPs/α-Fe <subscript>2</subscript> O <subscript>3</subscript> could partially absorb visible-light, which dramatically promotes electron transfer because of the excellent conductivity of Au NPs, and effectively inhibits the electron-hole recombination, thanks to the effect of heterostructures. Second, the signal-on type is based on the signal amplification label of GO/CdSe@CdS QDs which on adequate absorption of visible-light can improve generation of photogenerated electron-hole pairs effectively. Under optimal conditions, the well-prepared PEC immunosensor exhibited a low detection limit of 0.02 pg mL <superscript>-1</superscript> and a wide linear range from 0.06 pg mL <superscript>-1</superscript> to 350 ng mL <superscript>-1</superscript> for Aβ detection. Meanwhile, it also presented good reproducibility, specificity, and stability and might open a new promising pathway for detection of other important biomarkers.
- Subjects :
- Alzheimer Disease diagnosis
Amyloid beta-Peptides immunology
Antibodies chemistry
Cadmium Compounds chemistry
Electrochemical Techniques
Electrodes
Ferrosoferric Oxide chemistry
Gold chemistry
Humans
Limit of Detection
Metal Nanoparticles chemistry
Selenium Compounds chemistry
Sulfides chemistry
Amyloid beta-Peptides analysis
Antibodies immunology
Biosensing Techniques methods
Graphite chemistry
Quantum Dots chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 7
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 32254782
- Full Text :
- https://doi.org/10.1039/c8tb03164a