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Isatis tinctoria L.-derived Petroleum Ether Extract Mediates Anti-inflammatory Effects via Inhibition of Interleukin-6, Interleukin-33 and Mast Cell Degranulation.
- Source :
-
Acta dermato-venereologica [Acta Derm Venereol] 2020 May 11; Vol. 100 (10), pp. adv00131. Date of Electronic Publication: 2020 May 11. - Publication Year :
- 2020
-
Abstract
- Isatis tinctoria L. (woad) has been used in medicine for centuries and has demonstrated anti-inflammatory effects. However, to date, no well-defined extracts with precise analysis of active substances have been developed. The aim of this study was to develop novel extracts of Isatis tinctoria L., and to characterize their active ingredients and anti-inflammatory properties. Various extracts of Isatis tinctoria L. were analysed for their active ingredients, and screened for anti-inflammatory effects using cyclooxygenase-2 activity assays. A petroleum ether extract was found to have the best effects, and was tested in a mouse model of acute allergic contact dermatitis. In the mouse model the petroleum ether extract resulted in significantly reduced ear swelling, oedema and inflammatory cell density. In mouse skin and human keratinocyte cultures, petroleum ether extract inhibited pro-inflammatory cytokine expression. Furthermore, human mast cell degranulation was significantly inhibited in LAD2 cell cultures. In conclusion, novel woad extracts were developed and shown to have anti-inflammatory properties in a contact hypersensitivity animal model and human keratinocytes. The production of such extracts and further characterization of their specific properties will enable determination of their potential dermatological effects in the treatment of inflamed and irritated skin.
- Subjects :
- Administration, Topical
Animals
Anti-Inflammatory Agents administration & dosage
Anti-Inflammatory Agents immunology
Cells, Cultured
Dermatitis, Allergic Contact immunology
Dermatologic Agents administration & dosage
Dermatologic Agents immunology
Dermatologic Agents therapeutic use
Disease Models, Animal
Ear
Humans
Interleukin-1beta antagonists & inhibitors
Interleukin-1beta immunology
Interleukin-33 antagonists & inhibitors
Interleukin-33 immunology
Interleukin-6 antagonists & inhibitors
Interleukin-6 immunology
Keratinocytes drug effects
Keratinocytes immunology
Mast Cell Stabilizers administration & dosage
Mast Cell Stabilizers immunology
Mast Cell Stabilizers therapeutic use
Mast Cells drug effects
Mast Cells immunology
Mice
Plant Extracts administration & dosage
Plant Extracts pharmacology
Skin drug effects
Skin immunology
Skin pathology
Anti-Inflammatory Agents therapeutic use
Dermatitis, Allergic Contact drug therapy
Isatis
Phytotherapy
Plant Extracts therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1651-2057
- Volume :
- 100
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Acta dermato-venereologica
- Publication Type :
- Academic Journal
- Accession number :
- 32250439
- Full Text :
- https://doi.org/10.2340/00015555-3476