Back to Search
Start Over
A feasible strategy based on high ultrasound frequency and mass spectrometry for discriminating individuals diagnosed with bipolar disorder and schizophrenia through ionomic profile.
- Source :
-
Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2020 Sep; Vol. 34 Suppl 3, pp. e8798. Date of Electronic Publication: 2020 May 05. - Publication Year :
- 2020
-
Abstract
- Rationale: A viable and accurate method based on high-power ultrasound-assisted microextraction and inductively coupled plasma mass spectrometry was developed to determine metals in human serum from patients with bipolar disorder and schizophrenia.<br />Methods: A simple and rapid sample preparation method using a cup-horn sonoreactor was developed. The acid concentration of HNO <subscript>3</subscript> (10, 20, and 40% v/v) and HCl (1, 5, 15, and 30% v/v) of the extraction solution, the sonication time (1, 3, 6, and 10 min), and the sonication amplitude (20, 40, 60, and 80%) were evaluated. Cd, Cu, Fe, Li, Pb, and Zn were determined in serum samples from patients with bipolar disorder and schizophrenia, and from healthy controls. Quantitative metal recoveries using the proposed method were compared under the same conditions using an ultrasonic bath, magnetic stirring, and microwave-assisted digestion.<br />Results: Optimum extraction conditions were obtained using HNO <subscript>3</subscript> (40% v/v) + HCl (30% v/v) as the extraction solution with 3 min sonication time and 60% sonication amplitude. Significant differences were observed among the methods compared. On application of the sample preparation method based on high-power ultrasound-assisted microextraction coupled with inductively coupled plasma mass spectrometry, Pb and Cd in all the studied samples were below the limit of detection of our method. Compared with healthy controls, the concentration of Cu, Li, Fe, and Zn was found to be significantly higher for the bipolar disorder group, while these metals and Li were found at a lower level for the group diagnosed with schizophrenia.<br />Conclusions: Principal component analysis showed a significant separation for the groups studied based on their ionomic profiles after the application of high-power ultrasound-assisted microextraction as a sample preparation strategy.<br /> (© 2020 John Wiley & Sons, Ltd.)
- Subjects :
- Adult
Case-Control Studies
Chemical Fractionation instrumentation
Chemical Fractionation methods
Feasibility Studies
Female
Humans
Male
Microwaves
Middle Aged
Proof of Concept Study
Time Factors
Trace Elements blood
Ultrasonics
Bipolar Disorder blood
Blood Chemical Analysis methods
Mass Spectrometry methods
Metals blood
Schizophrenia blood
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0231
- Volume :
- 34 Suppl 3
- Database :
- MEDLINE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Publication Type :
- Academic Journal
- Accession number :
- 32246870
- Full Text :
- https://doi.org/10.1002/rcm.8798