Back to Search
Start Over
Effects of available phosphorus source and concentration on performance and expression of sodium phosphate type IIb cotransporter, vitamin D-1α-hydroxylase, and vitamin D-24-hydroxylase mRNA in broiler chicks.
- Source :
-
Poultry science [Poult Sci] 2020 Apr; Vol. 99 (4), pp. 1822-1831. Date of Electronic Publication: 2020 Feb 28. - Publication Year :
- 2020
-
Abstract
- This experiment was conducted to examine the effect of 2 phosphorus (P) sources on broiler performance to day 14. The P bioavailability was estimated using bird performance and tibia ash measurements, whereas P digestibility, intestinal P transporter, kidney vitamin D-1α-hydroxylase, and vitamin D-24-hydroxylase mRNA abundances were also determined. Slope regression analysis was used to determine the bioavailability of dicalcium phosphate (Dical P) and nanocalcium phosphate (Nano P) with dietary available P (AvP) set to 0.20% P (control) using AvP from the major ingredients and Dical P. The experimental treatments were achieved by supplementation with either Dical P or Nano P to generate 0.24, 0.28, 0.32, and 0.36% AvP. A total of 648-day-old unsexed broiler chicks were divided into 72 birds per treatment (8 replicate cages of 9 birds). Slope regression analysis showed positive linear relationships between BW, feed intake (FI), tibia ash weight (TAW), and tibia ash percentage (TAP) with dietary Dical P and Nano P levels. Comparisons between regression slopes for Dical P and Nano P fed birds were not significantly different for BW, feed intake, tibia ash weight, and tibia ash percentage, indicating similar P bioavailability from Dical P and Nano P. There were interactions between P source and AvP for feed efficiency (FE) and apparent ileal P digestibility (AIPD). Dicalcium phosphate had greater FE than Nano P at 0.28% AvP and greater AIPD than Nano P at 0.24% AvP. The addition of AvP from Dical P and Nano P resulted in reduced sodium phosphate cotransporter mRNA abundance in the duodenum in a dose-dependent response. In the kidney, vitamin D-1α-hydroxylase mRNA abundance was greater at 0.36% Nano P compared with control, but there was no difference with Dical P. There was no difference in vitamin D-24-hydroxylase mRNA abundance between control and supplementation with Nano P or Dical P. In conclusion, Nano P and Dical P had the same bioavailability but had different effects on gene expression.<br /> (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animal Feed analysis
Animals
Avian Proteins metabolism
Biological Availability
Calcium Phosphates administration & dosage
Calcium Phosphates pharmacokinetics
Chickens growth & development
Diet veterinary
Dietary Supplements analysis
Dose-Response Relationship, Drug
Duodenum metabolism
Kidney metabolism
Nanoparticles administration & dosage
Nanoparticles metabolism
Phosphorus, Dietary administration & dosage
Phosphorus, Dietary pharmacokinetics
RNA, Messenger metabolism
Random Allocation
Sodium-Phosphate Cotransporter Proteins, Type IIb metabolism
Steroid Hydroxylases metabolism
Vitamin D3 24-Hydroxylase metabolism
Avian Proteins genetics
Chickens genetics
Phosphorus, Dietary metabolism
Sodium-Phosphate Cotransporter Proteins, Type IIb genetics
Steroid Hydroxylases genetics
Vitamin D3 24-Hydroxylase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3171
- Volume :
- 99
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 32241462
- Full Text :
- https://doi.org/10.1016/j.psj.2019.12.035