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Sulfoxaflor Degraded by Aminobacter sp. CGMCC 1.17253 through Hydration Pathway Mediated by Nitrile Hydratase.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2020 Apr 22; Vol. 68 (16), pp. 4579-4587. Date of Electronic Publication: 2020 Apr 08. - Publication Year :
- 2020
-
Abstract
- Sulfoxaflor, a sulfoximine insecticide, could efficiently control many insect pests of sap-feeding. Microbial degradation of sulfoxaflor and the enzymatic mechanism involved have not been studied to date. A bacterial isolate JW2 that transforms sulfoxaflor to X11719474 was isolated and identified as Aminobacter sp. CGMCC 1.17253. Both the recombinant Escherichia coli strain harboring the Aminobacter sp. CGMCC 1.17253 nitrile hydratase (NHase) gene and the pure NHase acquired sulfoxaflor-degrading ability. Aminobacter sp. CGMCC 1.17253 NHase is a typical cobalt-containing NHase content of subunit α, subunit β, and an accessory protein, and the three-dimensional homology model of NHase was built. Substrate specificity tests showed that NHase catalyzed the conversion of acetamiprid, thiacloprid, indolyl-3-acetonitrile, 3-cyanopyridine, and benzonitrile into their corresponding amides, indicating its broad substrate specificity. This is the first report of the pure bacteria degradation of the sulfoxaflor residual in the environment and reveals the enzymatic mechanism mediated by Aminobacter sp. CGMCC 1.17253.
- Subjects :
- Bacterial Proteins genetics
Biodegradation, Environmental
Hydro-Lyases genetics
Insecticides chemistry
Phyllobacteriaceae enzymology
Phyllobacteriaceae genetics
Pyridines chemistry
Sulfur Compounds chemistry
Bacterial Proteins metabolism
Hydro-Lyases metabolism
Insecticides metabolism
Phyllobacteriaceae metabolism
Pyridines metabolism
Sulfur Compounds metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 68
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32227888
- Full Text :
- https://doi.org/10.1021/acs.jafc.9b06668