Streptobacillus canis sp. nov. isolated from a dog.

From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19 ^T was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4 % to the type strains of Streptobacillus felis , Streptobacillus notomytis , Streptobacillus ratti , Streptobacillus moniliformis and Streptobacillus hongkongensis , respectively. Strain IHIT1603-19 ^T could also clearly be differentiated from other Streptobacillus species by rpoB , groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA-DNA relatedness, strain IHIT1603-19 ^T demonstrated an average nucleotide identity of 83.00 and 82.28 % compared to S. felis 131000547 ^T and S. moniliformis DSM 12112 ^T , respectively. Chemotaxonomic and physiological data of strain IHIT1603-19 ^T were in congruence with other closely related members of the family Leptotrichiaceae , represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain IHIT1603-19 ^T from all currently described taxa of the genus Streptobacillus . On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19 ^T (=DSM 110501 ^T =CCUG 74118 ^T =CIP 111795 ^T ). The G+C content of the DNA of the type strain is 26.6 mol%, genome size is 1.60 Mbp.