Insilico study of genes involved in Congenital Hypothyroidism.

Authors :: Javed M
Masood A
Baig I
Mubeen H
Wattoo JI
Source :: JPMA. The Journal of the Pakistan Medical Association [J Pak Med Assoc] 2020 Mar; Vol. 70 (3), pp. 427-431.
Publication Year :: 2020
Abstract: Objective: To study the orthologs of the five genes of congenital hypothyroidism NIS, PAX8, DUOX2, FOXE1, NKX2-1 that are involved in the development of the thyroid gland.<br />Methods: The study was conducted at INMOL Cancer Hospital, Lahore in September 2017 and comprised of finding gene orthologs, phylogenetic tree and domains of NIS, PAX8, DUOX2, FOXE1, NKX2-1 which were studied using different bioinformatics tools, including FASTA, BLAST, ENSEMBL, UniProt, MultiAlin, to find out the important domains involved in the mutations of these genes.<br />Results: Genes showed consensus sequence / motifs involved in congenital hypothyroidism. Phylogenetic results showed that these genes shared some common motifs. Phylogenetic trees revealed sub-clusters with high protein homology.<br />Conclusions: Genes involved in congenital hypothyroidism were found to have a consensus sequence motifs.