Back to Search
Start Over
Superior adjuvanticity of the genetically fused D1 domain of Neisseria meningitides Ag473 lipoprotein among three Toll-like receptor ligands.
- Source :
-
Bioscience reports [Biosci Rep] 2020 Apr 30; Vol. 40 (4). - Publication Year :
- 2020
-
Abstract
- Toll-like receptor (TLR) ligands have emerged as the attractive adjuvant for subunit vaccines. However, selection of TLR ligands needs to be rationally chosen on the basis of antigen and adjuvant properties. In the present study, we expressed the Ag473 lipoprotein from Neisseria meningitides, flagellin FlaB from Vibrio vulnificus and heat shock protein 70 from Mycobacterium tuberculosis (mHsp70) in Escherichia coli as single proteins and fusion proteins with VP2 protein of infectious bursal disease virus (IBDV). Both cellular and humoral adjuvanticities of the three TLR ligands were compared by immunization of mice in two different ways. Among the three co-administered TLR ligands, recombinant Ag473 lipoprotein exhibited the highest cellular and humoral adjuvanticities, including promotion of IL-4, IL-12, IFN-γ and IBDV VP2-specific antibody production. Among the three genetically fused TLR ligands, fusion with Ag473 D1 domain exhibited the highest cellular and humoral adjuvanticities. Overall, the adjuvanticities of genetically fused TRL ligands were significantly higher than that of co-administered TLR ligands. Fusion with Ag473 D1 domain exhibited superior adjuvanticity among the three TLR ligands delivered in two different ways.<br /> (© 2020 The Author(s).)
- Subjects :
- Animals
Bacterial Proteins administration & dosage
Bacterial Proteins genetics
Female
Flagellin administration & dosage
Flagellin genetics
Flagellin immunology
HSP70 Heat-Shock Proteins administration & dosage
HSP70 Heat-Shock Proteins genetics
HSP70 Heat-Shock Proteins immunology
Ligands
Mice
Models, Animal
Protein Domains genetics
Protein Domains immunology
Recombinant Fusion Proteins administration & dosage
Recombinant Fusion Proteins genetics
Vaccines, Subunit administration & dosage
Vaccines, Subunit genetics
Vibrio vulnificus genetics
Vibrio vulnificus immunology
Viral Structural Proteins administration & dosage
Viral Structural Proteins genetics
Viral Structural Proteins immunology
Bacterial Proteins immunology
Immunogenicity, Vaccine
Recombinant Fusion Proteins immunology
Vaccines, Subunit immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-4935
- Volume :
- 40
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience reports
- Publication Type :
- Academic Journal
- Accession number :
- 32202301
- Full Text :
- https://doi.org/10.1042/BSR20193675