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Ski Is Required for Tri-Methylation of H3K9 in Major Satellite and for Repression of Pericentromeric Genes: Mmp3, Mmp10 and Mmp13, in Mouse Fibroblasts.
- Source :
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Journal of molecular biology [J Mol Biol] 2020 May 01; Vol. 432 (10), pp. 3222-3238. Date of Electronic Publication: 2020 Mar 19. - Publication Year :
- 2020
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Abstract
- Several mechanisms directing a rapid transcriptional reactivation of genes immediately after mitosis have been described. However, little is known about the maintenance of repressive signals during mitosis. In this work, we address the role of Ski in the repression of gene expression during M/G <subscript>1</subscript> transition in mouse embryonic fibroblasts (MEFs). We found that Ski localises as a distinct pair of dots at the pericentromeric region of mitotic chromosomes, and the absence of the protein is related to high acetylation and low tri-methylation of H3K9 in pericentromeric major satellite. Moreover, differential expression assays in early G <subscript>1</subscript> cells showed that the presence of Ski is significantly associated with repression of genes localised nearby to pericentromeric DNA. In mitotic cells, chromatin immunoprecipitation assays confirmed the association of Ski to major satellite and the promoters of the most repressed genes: Mmp3, Mmp10 and Mmp13. These genes are at pericentromeric region of chromosome 9. In these promoters, the presence of Ski resulted in increased H3K9 tri-methylation levels. This Ski-dependent regulation is also observed during interphase. Consequently, Mmp activity is augmented in Ski-/- MEFs. Altogether, these data indicate that association of Ski with the pericentromeric region of chromosomes during mitosis is required to maintain the silencing bookmarks of underlying chromatin.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Acetylation
Animals
Cells, Cultured
Centromere metabolism
Down-Regulation
Fibroblasts metabolism
Matrix Metalloproteinase 10 genetics
Matrix Metalloproteinase 13 genetics
Matrix Metalloproteinase 3 genetics
Methylation
Mice
Mitosis
Promoter Regions, Genetic
Transcriptional Activation
Centromere genetics
DNA-Binding Proteins metabolism
Fibroblasts cytology
Histones metabolism
Matrix Metalloproteinases, Secreted genetics
Proto-Oncogene Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8638
- Volume :
- 432
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 32198114
- Full Text :
- https://doi.org/10.1016/j.jmb.2020.03.013