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A Co-registration Pipeline for Multimodal MALDI and Confocal Imaging Analysis of Stem Cell Colonies.
- Source :
-
Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2020 Apr 01; Vol. 31 (4), pp. 986-989. Date of Electronic Publication: 2020 Mar 19. - Publication Year :
- 2020
-
Abstract
- Multimodal mass spectrometry imaging (MSI) data presents unique big data challenges in handling and analysis. Here, we present a pipeline for co-registering matrix-assisted laser desorption/ionization MSI and confocal immunofluorescence imaging data for extracting single-cell metabolite signatures. We further describe methods and introduce software for the simultaneous analysis of these concatenated data sets, which are designed to establish a connection between cell traits of interest (shape metrics, position within sample) and the cells' own metabolic signatures.
Details
- Language :
- English
- ISSN :
- 1879-1123
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of the American Society for Mass Spectrometry
- Publication Type :
- Academic Journal
- Accession number :
- 32176489
- Full Text :
- https://doi.org/10.1021/jasms.9b00094