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Quantification of [ 11 C]PBR28 data after systemic lipopolysaccharide challenge.
- Source :
-
EJNMMI research [EJNMMI Res] 2020 Mar 12; Vol. 10 (1), pp. 19. Date of Electronic Publication: 2020 Mar 12. - Publication Year :
- 2020
-
Abstract
- Background: Lipopolysaccharide (LPS) is a classic immune stimulus. LPS combined with positron emission tomography (PET) 18 kDa translocator protein (TSPO) brain imaging provides a robust human laboratory model to study neuroimmune signaling. To evaluate optimal analysis of these data, this work compared the sensitivity of six quantification approaches.<br />Methods: [ <superscript>11</superscript> C]PBR28 data from healthy volunteers (N = 8) were collected before and 3 h after LPS challenge (1.0 ng/kg IV). Quantification approaches included total volume of distribution estimated with two tissue, and two tissue plus irreversible uptake in whole blood, compartment models (2TCM and 2TCM-1k, respectively) and multilinear analysis-1 (MA-1); binding potential estimated with simultaneous estimation (SIME); standardized uptake values (SUV); and SUV ratio (SUVR).<br />Results: The 2TCM, 2TCM-1k, MA-1, and SIME approaches each yielded substantive effect sizes for LPS effects (partial η <superscript>2</superscript> = 0.56-0.89, ps <. 05), whereas SUV and SUVR did not.<br />Conclusion: These findings highlight the importance of incorporating AIF measurements to quantify LPS-TSPO studies.
Details
- Language :
- English
- ISSN :
- 2191-219X
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- EJNMMI research
- Publication Type :
- Academic Journal
- Accession number :
- 32166497
- Full Text :
- https://doi.org/10.1186/s13550-020-0605-7