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Clinical value of tissue DNA integrity index in pancreatic cancer.
- Source :
-
The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland [Surgeon] 2020 Oct; Vol. 18 (5), pp. 269-279. Date of Electronic Publication: 2020 Mar 07. - Publication Year :
- 2020
-
Abstract
- Background: DNA integrity index as a blood biomarker is associated with the prognosis of cancer patients.<br />Aims: The primary goal of the study was to examine tissue DNA integrity index (DII) in a group of pancreatic cancer (PC) tumor tissues and control adjacent pancreatic tissues. We also aimed to test the relationship between the tumor tissue DII and the clinicopathological parameters and the overall survival.<br />Methods: In the prospective study, DII was calculated using: the Alu 247/115 ratio, the LINE1 300/79 ratio and the average of the above values, based on the data obtained by real-time PCR. The tumors samples (n = 42) originated from the patients with pathologically confirmed pancreatic ductal adenocarcinoma and the control adjacent pancreatic tissue specimens (n = 32) were received from surgical margins.<br />Results: Specimens from the tumors pathologically marked as R1 (microscopic residual tumor) had a significantly higher LINE1 300/79 ratio values than specimens from adjacent normal pancreatic tissue (P<0.05). ROC curve analysis revealed that LINE1 300/79 ratio is a good parameter to distinguish between R0 and R1 tumors (AUC = 0.703, P<0.05).<br />Conclusions: This is the first study exploring the tissue DNA integrity index (DII) in pancreatic cancer. LINE1 DII can be used as auxiliary parameter for objective evaluation of margin status.<br /> (Copyright © 2020. Published by Elsevier Ltd.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Alu Elements genetics
Biomarkers, Tumor
Carcinoma, Pancreatic Ductal pathology
Female
Humans
Male
Middle Aged
Pancreatic Neoplasms pathology
Prognosis
Prospective Studies
Sensitivity and Specificity
Survival Rate
Carcinoma, Pancreatic Ductal genetics
Carcinoma, Pancreatic Ductal mortality
DNA Fragmentation
Pancreatic Neoplasms genetics
Pancreatic Neoplasms mortality
Subjects
Details
- Language :
- English
- ISSN :
- 1479-666X
- Volume :
- 18
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
- Publication Type :
- Academic Journal
- Accession number :
- 32156475
- Full Text :
- https://doi.org/10.1016/j.surge.2019.10.008