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Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments of Mongolian horses.
- Source :
-
MicrobiologyOpen [Microbiologyopen] 2020 Jun; Vol. 9 (6), pp. 1085-1101. Date of Electronic Publication: 2020 Mar 09. - Publication Year :
- 2020
-
Abstract
- The intestinal microbiota plays an important role in the health and metabolism of the host. Next-generation sequencing technology has enabled the characterization of the gut microbiota of several animal species. We analyzed the intestinal microbiota in six different parts of the gastrointestinal tracts (GITs) of five Mongolian horses by sequencing the 16S rRNA gene V3-V4 hypervariable region. All horses were kept in the natural habitat of the Inner Mongolia grassland. Significant differences were observed among the microbiota compositions of the distinct GIT regions. In addition, while the microbial community structures of the small and large intestine were significantly different, those of the cecum and colon were similar. In the foregut, Firmicutes (65%) and Proteobacteria (23%) were the most abundant, while Firmicutes (45%) and Bacteroidetes (42%) were the most common in the hindgut. At the level of family, Ruminococcaceae (p = .203), Lachnospiraceae (p = .157), Rikenellaceae (p = .122), and Prevotellaceae (p = .068) were predominant in the hindgut, while the relative abundance of the Akkermansia genus (5.7%, p = .039) was higher in the ventral colon. In terms of the putative functions, the ratio of microbial abundance in the different parts of the GIT was similar, the result can help characterize the gut microbial structure of different animals.<br /> (© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
- Subjects :
- Akkermansia genetics
Akkermansia isolation & purification
Animals
Bacteroidetes genetics
Bacteroidetes isolation & purification
Cecum microbiology
China
Clostridiales genetics
Clostridiales isolation & purification
Colon microbiology
DNA, Bacterial genetics
Female
Firmicutes genetics
Firmicutes isolation & purification
Gastrointestinal Tract microbiology
High-Throughput Nucleotide Sequencing
Horses
Intestine, Large microbiology
Intestine, Small microbiology
Male
Proteobacteria genetics
Proteobacteria isolation & purification
RNA, Ribosomal, 16S genetics
Akkermansia classification
Bacteroidetes classification
Clostridiales classification
Firmicutes classification
Gastrointestinal Microbiome genetics
Proteobacteria classification
Subjects
Details
- Language :
- English
- ISSN :
- 2045-8827
- Volume :
- 9
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- MicrobiologyOpen
- Publication Type :
- Academic Journal
- Accession number :
- 32153142
- Full Text :
- https://doi.org/10.1002/mbo3.1020