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Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2118, pp. 111-120. - Publication Year :
- 2020
-
Abstract
- Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and D-phenylalanyl-L-prolyl-L-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.
- Subjects :
- Amino Acid Chloromethyl Ketones chemistry
Amino Acid Chloromethyl Ketones pharmacology
Antithrombins chemistry
Antithrombins pharmacology
Hirudins chemistry
Hirudins pharmacology
Magnetic Iron Oxide Nanoparticles
Nanoparticles
Particle Size
Peptide Fragments chemistry
Peptide Fragments pharmacology
Polyhydroxyethyl Methacrylate
Recombinant Proteins chemical synthesis
Recombinant Proteins chemistry
Recombinant Proteins pharmacology
Amino Acid Chloromethyl Ketones chemical synthesis
Antithrombins chemical synthesis
Fluorocarbons chemistry
Hirudins chemical synthesis
Peptide Fragments chemical synthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2118
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 32152974
- Full Text :
- https://doi.org/10.1007/978-1-0716-0319-2_8