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Comparative nephroprotective effects of curcumin and etoricoxib against cisplatin-induced acute kidney injury in rats.
- Source :
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Acta histochemica [Acta Histochem] 2020 May; Vol. 122 (4), pp. 151534. Date of Electronic Publication: 2020 Mar 06. - Publication Year :
- 2020
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Abstract
- Objective: Although cisplatin (CIS) acts as potent chemotherapy, nephrotoxicity still its major life-threatening side effect. The purpose of this study was to discuss and compare the renoprotective effects of curcumin (CUR) and etoricoxib (ETB) against CIS-induced nephrotoxicity.<br />Materials & Methods: Thirty six adult female rats were divided equally into 6 groups: Group I (control), Group II (CIS) received cisplatin (7.5 mg/kg i.p), Group III (CUR) and group IV (ETB) received curcumin (200 mg/kg/day) or etoricoxib (10 mg/kg/day) respectively via gavage for seven continuous days. Group V (CIS + CUR) and Group VI (CIS + ETB) received curcumin (200 mg/kg/day) or etoricoxib (10 mg/kg/day) via gavage for seven continuous days. On the 4th day, the rats received cisplatin (7.5 mg/kg i.p) as a single injection 1 h after last curcumin or etoricoxib administration. At the assigned time, blood and tissue samples were collected for biochemical, histochemical, histopathological, immunohistochemical, and RT-PCR gene expression studies.<br />Results: Curcumin administration significantly decreased CIS-induced elevation of serum creatinine and blood urea nitrogen (BUN), and reversed oxidative stress markers; glutathione (GSH) and malondialdehyde (MDA) to control level. Suppression of inflammatory and apoptotic responses by CUR co-treatment was evidenced by decreased iNOS and BAX immunohistochemical reactions, and TNF-α and Caspase3 gene expressions which were detected by RT-PCR in kidney tissues. To our knowledge, this is the first time to discuss the effect of ETB on CIS induced nephrotoxicity. Although ETB reduced the previously mentioned inflammatory and apoptotic markers, its effect was less than that of CUR. Administration of ETB couldn't modify the disturbed levels of creatinine, BUN, GSH, and MDA.<br />Conclusion: In conclusion, CUR provided a promising renoprotective effect against CIS induced nephrotoxicity. Further studies are recommended to approve or disapprove the protective role of ETB in CIS induced nephrotoxicity.<br />Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.<br /> (Copyright © 2020 Elsevier GmbH. All rights reserved.)
- Subjects :
- Acute Kidney Injury pathology
Animals
Apoptosis drug effects
Blood Urea Nitrogen
Creatinine blood
Female
Gene Expression Regulation drug effects
Glutathione metabolism
Malondialdehyde metabolism
Oxidative Stress drug effects
Rats
Acute Kidney Injury chemically induced
Acute Kidney Injury prevention & control
Anti-Inflammatory Agents, Non-Steroidal pharmacology
Antineoplastic Agents toxicity
Cisplatin toxicity
Curcumin pharmacology
Cyclooxygenase 2 Inhibitors pharmacology
Etoricoxib pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0372
- Volume :
- 122
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Acta histochemica
- Publication Type :
- Academic Journal
- Accession number :
- 32151374
- Full Text :
- https://doi.org/10.1016/j.acthis.2020.151534