Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil.

Authors :: Dos Santos Silva AC
de Barros LD
Barros VMC
de Alcântara AM
Andrade MR
Garcia JL
Mota RA
Porto WJN
Source :: Acta parasitologica [Acta Parasitol] 2020 Sep; Vol. 65 (3), pp. 774-778. Date of Electronic Publication: 2020 Mar 05.
Publication Year :: 2020
Abstract: Purpose: The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil.<br />Methods: Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR-RFLP.<br />Results: Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database.<br />Conclusions: Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.

Subjects :: Animals
Antibodies, Protozoan blood
Biological Assay
Brazil epidemiology
DNA, Protozoan genetics
Food Microbiology
Genetic Variation
Genotype
Humans
Mice
Poultry Diseases parasitology
Toxoplasma classification
Chickens parasitology
Poultry parasitology
Poultry Diseases epidemiology
Toxoplasma genetics
Toxoplasmosis, Animal epidemiology

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