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Oxidative stress enhanced the transforming growth factor-β2-induced epithelial-mesenchymal transition through chemokine ligand 1 on ARPE-19 cell.
- Source :
-
Scientific reports [Sci Rep] 2020 Mar 04; Vol. 10 (1), pp. 4000. Date of Electronic Publication: 2020 Mar 04. - Publication Year :
- 2020
-
Abstract
- Fibroblast-like transformation of retinal pigment epithelial (RPE) cells is a pathological feature of proliferative vitreoretinopathy (PVR) that may cause blindness. The effect of oxidative stress alone or together with transforming growth factor-beta 2 (TGF-β2) on epithelial-mesenchymal transformation (EMT) is not fully understood in RPE. TGF-β2 induced the upregulation EMT markers including α-smooth muscle actin (α-SMA), Snail, and Slug and downregulation of E-cadherin (E-cad) in ARPE-19 cells. Hydrogen peroxide (H <subscript>2</subscript> O <subscript>2</subscript> ) not only upregulated α-SMA but also enhanced the effect of TGF-β2 on the expression of Snail and Slug. The CXCL family of cytokines could be the mediators of EMT induced by H <subscript>2</subscript> O <subscript>2</subscript> and TGF-β2. H <subscript>2</subscript> O <subscript>2</subscript> induced CXCL1, that upregulated α-SMA and fibronectin. Both SB225002, an inhibitor of CXCR2, and antioxidant N-acetylcysteine suppressed the TGF-β2-induced EMT in ARPE-19 cells. Taken together, the results suggest that oxidative stress enhanced TGF-β2-induced EMT through the possible autocrine effect of CXCL1 on CXCR2 in ARPE-19 cells.
- Subjects :
- Cell Line
Gene Expression Regulation drug effects
Humans
Hydrogen Peroxide pharmacology
Phenylurea Compounds pharmacology
Receptors, Interleukin-8B antagonists & inhibitors
Receptors, Interleukin-8B metabolism
Vitreoretinopathy, Proliferative metabolism
Vitreoretinopathy, Proliferative pathology
Autocrine Communication drug effects
Chemokine CCL1 biosynthesis
Epithelial-Mesenchymal Transition drug effects
Oxidative Stress drug effects
Transforming Growth Factor beta2 pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 32132577
- Full Text :
- https://doi.org/10.1038/s41598-020-60785-x