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Yangambin and Epi-yangambin Isomers: New Purification Method from Ocotea fasciculata and First Cytotoxic Aspects Focusing on In Vivo Safety.
- Source :
-
Planta medica [Planta Med] 2020 Apr; Vol. 86 (6), pp. 415-424. Date of Electronic Publication: 2020 Mar 03. - Publication Year :
- 2020
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Abstract
- Ocotea fasciculata presents yangambin (YAN) and its isomer epi-yangambin (EPI-YAN) as major lignans, which are employed as the plant markers for quality control purposes and as potential pharmacological compounds. However, a gap between the pure isomers and safety and efficacy protocols is faced by the scientific community. In this context, this work aimed to report (i) a new and advantageous purifying process in a semi-preparative scale for YAN and EPI-YAN isolation from Ocotea fasciculata , and (ii) an in vitro cytotoxicity study to estimate, for the first time, the LD <subscript>50</subscript> values of the isolated epimers, as well as the influence of albumin concentration in cell culture medium. The best condition for epimers isolation was achieved in normal-phase liquid chromatography. The lignan fraction (LF), previously obtained from the plant ethanolic extract, was purified yielding 17% and 29% of YAN and EPI-YAN, respectively. The in vitro study demonstrated that YAN and EPI-YAN were safe, and only at the highest concentration studied, a decrease on cell viability was observed. The estimated LD <subscript>50</subscript> value was higher than 1612 mg/kg for both epimers. The LF, on the other hand, demonstrated an estimated LD <subscript>50</subscript> of 422 mg/kg. Lignan cytotoxicity studies also evidenced that the higher cell viability was related to the higher concentration of fetal bovine serum as a source of albumin in medium. This is the first time the LD <subscript>50</subscript> and safety of the isolated epimers were estimated, opening up great perspectives of success in in vivo studies.<br />Competing Interests: The authors declare that they have no conflict of interest.<br /> (Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Plant Extracts
Furans
Lignans
Ocotea
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0221
- Volume :
- 86
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 32126582
- Full Text :
- https://doi.org/10.1055/a-1118-3828