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Alternatively Constructed Estrogen Receptor Alpha-Driven Super-Enhancers Result in Similar Gene Expression in Breast and Endometrial Cell Lines.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2020 Feb 27; Vol. 21 (5). Date of Electronic Publication: 2020 Feb 27. - Publication Year :
- 2020
-
Abstract
- Super-enhancers (SEs) are clusters of highly active enhancers, regulating cell type-specific and disease-related genes, including oncogenes. The individual regulatory regions within SEs might be simultaneously bound by different transcription factors (TFs) and co-regulators, which together establish a chromatin environment conducting to effective transcription. While cells with distinct TF profiles can have different functions, how different cells control overlapping genetic programs remains a question. In this paper, we show that the construction of estrogen receptor alpha-driven SEs is tissue-specific, both collaborating TFs and the active SE components greatly differ between human breast cancer-derived MCF-7 and endometrial cancer-derived Ishikawa cells; nonetheless, SEs common to both cell lines have similar transcriptional outputs. These results delineate that despite the existence of a combinatorial code allowing alternative SE construction, a single master regulator might be able to determine the overall activity of SEs.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Basic Helix-Loop-Helix Transcription Factors genetics
Basic Helix-Loop-Helix Transcription Factors metabolism
Binding Sites genetics
Breast Neoplasms genetics
Cell Line, Tumor
Chromatin Immunoprecipitation Sequencing
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
E-Box Elements genetics
Endometrial Neoplasms genetics
Endometrium cytology
Estrogen Receptor alpha genetics
Female
Forkhead Box Protein M1 genetics
Forkhead Box Protein M1 metabolism
Gene Expression Regulation, Neoplastic genetics
Humans
MCF-7 Cells
Muscle Proteins genetics
Muscle Proteins metabolism
TEA Domain Transcription Factors
Transcription Factors genetics
Transcriptome
Breast Neoplasms metabolism
Endometrial Neoplasms metabolism
Endometrium metabolism
Enhancer Elements, Genetic
Estrogen Receptor alpha metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 32120995
- Full Text :
- https://doi.org/10.3390/ijms21051630