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Effects of 27 natural products on drug metabolism genes in channel catfish ( Ictalurus punctatus ) cell line.
- Source :
-
Xenobiotica; the fate of foreign compounds in biological systems [Xenobiotica] 2020 Sep; Vol. 50 (9), pp. 1043-1051. Date of Electronic Publication: 2020 Mar 12. - Publication Year :
- 2020
-
Abstract
- Pregnane X receptor (PXR) as a ligand dependent transcription factor, is capable of regulating gene expression of cytochromes P450 and transporters involved in xenobiotic/drug metabolism and elimination. Due to the species differences in the regulatory specificity of PXR, gene regulation should not be extrapolated from mammal to fish without research data.The aim of present study was to investigate the effect of 27 natural products on PXR, CYP3A30 and MDR1 genes in channel catfish ( Ietalurus punetaus ) kidney cells (CC-K). The results showed that bisdemethoxycurcumin, glycyrrhetnic acid, rotenone, artemisinin, dihydroartemisinin, ligustilide and matrine strongly induced the mRNA levels of PXR. Additionally, the up-regulation of CYP3A30 gene ran parallel with PXR gene after the treatment of demethoxycurcumin, glycyrrhetnic acid, artemisinin, matrine, baicalein, schisantherin A, ligustilide, and dihydroartemisinin. Moreover, we found that natural products schisandrin A, schisandrin B, schisandrol A, and schisandrol B significantly up-regulated the mRNA level of MDR1 gene.Our work with a view to provide experimental data support for further research, which will make for the rational application of natural products in channel catfish, such as to avoid adverse herb-drug interactions or accelerating the residue elimination of chemical medicine.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Animals
Biological Products metabolism
Cell Line
Cyclooctanes metabolism
Cyclooctanes pharmacology
Cytochrome P-450 Enzyme System metabolism
Dioxoles metabolism
Dioxoles pharmacology
Ictaluridae
Lignans metabolism
Lignans pharmacology
Polycyclic Compounds metabolism
Polycyclic Compounds pharmacology
Pregnane X Receptor metabolism
Biological Products pharmacology
Biotransformation genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1366-5928
- Volume :
- 50
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Xenobiotica; the fate of foreign compounds in biological systems
- Publication Type :
- Academic Journal
- Accession number :
- 32118504
- Full Text :
- https://doi.org/10.1080/00498254.2020.1737760