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Effects of the Positive Threshold and Data Analysis on Human MOG Antibody Detection by Live Flow Cytometry.
- Source :
-
Frontiers in immunology [Front Immunol] 2020 Feb 06; Vol. 11, pp. 119. Date of Electronic Publication: 2020 Feb 06 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Human autoantibodies targeting myelin oligodendrocyte glycoprotein (MOG Ab) have become a useful clinical biomarker for the diagnosis of a spectrum of inflammatory demyelinating disorders. Live cell-based assays that detect MOG Ab against conformational MOG are currently the gold standard. Flow cytometry, in which serum binding to MOG-expressing cells and control cells are quantitively evaluated, is a widely used observer-independent, precise, and reliable detection method. However, there is currently no consensus on data analysis; for example, seropositive thresholds have been reported using varying standard deviations above a control cohort. Herein, we used a large cohort of 482 sera including samples from patients with monophasic or relapsing demyelination phenotypes consistent with MOG antibody-associated demyelination and other neurological diseases, as well as healthy controls, and applied a series of published analyses involving a background subtraction (delta) or a division (ratio). Loss of seropositivity and reduced detection sensitivity were observed when MOG ratio analyses or when 10 standard deviation (SD) or an arbitrary number was used to establish the threshold. Background binding and MOG ratio value were negatively correlated, in which patients seronegative by MOG ratio had high non-specific binding, a characteristic of serum that must be acknowledged. Most MOG Ab serostatuses were similar across analyses when optimal thresholds obtained by ROC analyses were used, demonstrating the robust nature and high discriminatory power of flow cytometry cell-based assays. With increased demand to identify MOG Ab-positive patients, a consensus on analysis is vital to improve patient diagnosis and for cross-study comparisons to ultimately define MOG Ab-associated disorders.<br /> (Copyright © 2020 Tea, Pilli, Ramanathan, Lopez, Merheb, Lee, Zou, Liyanage, Bassett, Thomsen, Reddel, Barnett, Brown, Dale, Brilot and the Australasian New Zealand MOG Study Group.)
- Subjects :
- Adult
Biomarkers analysis
Child
Cohort Studies
Data Analysis
Demyelinating Diseases diagnosis
Demyelinating Diseases immunology
Female
Healthy Volunteers
Humans
Male
Middle Aged
Nervous System Diseases diagnosis
Nervous System Diseases immunology
Retrospective Studies
Serum
Autoantibodies immunology
Autoantibodies metabolism
Flow Cytometry statistics & numerical data
Myelin-Oligodendrocyte Glycoprotein immunology
Myelin-Oligodendrocyte Glycoprotein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 32117270
- Full Text :
- https://doi.org/10.3389/fimmu.2020.00119