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Stem rust resistance in wheat is suppressed by a subunit of the mediator complex.
- Source :
-
Nature communications [Nat Commun] 2020 Feb 28; Vol. 11 (1), pp. 1123. Date of Electronic Publication: 2020 Feb 28. - Publication Year :
- 2020
-
Abstract
- Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
- Subjects :
- Basidiomycota pathogenicity
Chromosome Mapping
Chromosomes, Plant genetics
Gene Duplication
Gene Expression
Gene Expression Regulation, Plant
Genes, Plant genetics
Mutation
Phenotype
Plant Diseases immunology
Plant Diseases microbiology
Plant Immunity genetics
Poaceae classification
Poaceae genetics
Triticum immunology
Triticum microbiology
Disease Resistance genetics
Mediator Complex genetics
Plant Diseases genetics
Triticum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 32111840
- Full Text :
- https://doi.org/10.1038/s41467-020-14937-2