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In vitro safety of ketotifen as a topical nasal rinse.

Authors :
Triantafillou V
Maina IW
Patel NN
Tong CCL
Papagiannopoulos P
Kohanski MA
Kennedy DW
Palmer JN
Adappa ND
Cohen NA
Bosso JV
Source :
International forum of allergy & rhinology [Int Forum Allergy Rhinol] 2020 Feb; Vol. 10 (2), pp. 265-270.
Publication Year :
2020

Abstract

Background: Ketotifen is a second-generation noncompetitive H1-antihistamine and mast-cell stabilizer. It is commonly used to treat or prevent allergic conjunctivitis, asthma, chronic urticaria, anaphylaxis, mast-cell, and other allergic-type disorders. However, it has never been studied in aspirin-exacerbated respiratory disease (AERD), an aggressive phenotype of chronic rhinosinusitis with nasal polyps, where the mast cell plays a prominent role its pathogenesis.<br />Methods: Human sinonasal epithelial cells were grown at an air-liquid interface (ALI). Ketotifen powder was dissolved in saline to make 4 test solutions at 1.04, 2.08, 10.4, and 20.8 µg/mL. Control (saline) or ketotifen solution was added apically to ALI cultures from tissue of 5 unique patients, and ciliary beat frequency (CBF) changes were recorded. Lactate dehydrogenase was measured at 24 and 48 hours to estimate long-term cellular toxicity.<br />Results: Apical application of ketotifen at all concentrations was neither ciliotoxic nor ciliostimulatory, with no change in CBF over a period of 15 minutes after application. Cellular toxicity for all concentrations at 24 and 48 hours after application was <3% and <7%, respectively, that of lysed cultures.<br />Conclusion: Topical application of ketotifen to an in vitro model of sinonasal epithelium is safe, as evaluated by CBF and lactate dehydrogenase. Ketotifen is neither ciliotoxic nor ciliostimulatory, and no long-term cellular toxicity was observed. Ketotifen may have promise as a topical nasal rinse in the treatment of AERD.<br /> (© 2019 ARS-AAOA, LLC.)

Details

Language :
English
ISSN :
2042-6984
Volume :
10
Issue :
2
Database :
MEDLINE
Journal :
International forum of allergy & rhinology
Publication Type :
Academic Journal
Accession number :
32086998
Full Text :
https://doi.org/10.1002/alr.22461