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Schizandrin attenuates inflammation induced by avian pathogenic Escherichia coli in chicken type II pneumocytes.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2020 Apr; Vol. 81, pp. 106313. Date of Electronic Publication: 2020 Feb 15. - Publication Year :
- 2020
-
Abstract
- Avian pathogenic Escherichia coli (APEC) is a kind of highly pathogenic parenteral bacteria, which adheres to chicken type II pneumocytes through pili, causing inflammatory damage of chicken type II pneumocytes. Without affecting the growth of bacteria, anti-adhesion to achieve anti-inflammatory effect is considered to be a new method for the treatment of multi-drug-resistant bacterial infections. In this study, the anti-APEC activity of schizandrin was studied in vitro. By establishing the model of chicken type II pneumocytes infected with APEC-O78, the adhesion number, the expression of virulence genes, the release of lactate dehydrogenase (LDH), levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8 and activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways were detected. The results showed that schizandrin reduced the release of LDH and the adherence of APEC on chicken type II pneumocytes. Moreover, schizandrin markedly decreased the levels of IL-1β, IL-8, IL-6, and TNF-α, the mechanism responsible for these effects was attributed to the inhibitory effect of schizandrin on NF-κB and MAPK signaling activation. In conclusion, our findings revealed that schizandrin could reduce the inflammatory injury of chicken type II pneumocytes by reducing the adhesion of APEC-O78 to chicken type II pneumocytes. The results indicate that schizandrin can be a potential agent to treat inflammation caused by avian colibacillosis.<br /> (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Bacterial Adhesion
Cells, Cultured
Chickens
Cytokines metabolism
Extracellular Signal-Regulated MAP Kinases metabolism
Inflammation Mediators metabolism
L-Lactate Dehydrogenase metabolism
NF-kappa B metabolism
Signal Transduction
Alveolar Epithelial Cells physiology
Anti-Inflammatory Agents therapeutic use
Cyclooctanes therapeutic use
Escherichia coli physiology
Escherichia coli Infections drug therapy
Inflammation drug therapy
Lignans therapeutic use
Polycyclic Compounds therapeutic use
Poultry Diseases drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 81
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 32070918
- Full Text :
- https://doi.org/10.1016/j.intimp.2020.106313