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A high affinity modified DNA aptamer containing base-appended bases for human β-defensin.
- Source :
-
Analytical biochemistry [Anal Biochem] 2020 Apr 01; Vol. 594, pp. 113627. Date of Electronic Publication: 2020 Feb 15. - Publication Year :
- 2020
-
Abstract
- We used base-appended base modification to develop a new adenine analog, which incorporates an adenine derivative at position 7 of adenine. Using the systematic evolution of ligands by exponential enrichment method with a modified DNA library including this analog, we obtained A <superscript>ad1</superscript> , an aptamer that binds strongly to human β-defensin 2, a biomarker of physical stress found in saliva. The dissociation constant of A <superscript>ad1</superscript> with respect to human β-defensin 2 was found to be low (6.8 nM), and was found to bind specifically to human β-defensin 2 in saliva spiked with the protein, as confirmed using pull-down with magnetic beads. To our knowledge, there are no prior reports of nucleic-acid aptamers that bind specifically to human β-defensin 2. However, our results indicated that such adenine analog-containing DNA libraries are extremely effective in the acquisition of high-affinity aptamers.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1096-0309
- Volume :
- 594
- Database :
- MEDLINE
- Journal :
- Analytical biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32067984
- Full Text :
- https://doi.org/10.1016/j.ab.2020.113627