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Rose Bengal-Mediated Photodynamic Antimicrobial Treatment of Acanthamoeba Keratitis.
- Source :
-
Current eye research [Curr Eye Res] 2020 Oct; Vol. 45 (10), pp. 1205-1210. Date of Electronic Publication: 2020 Feb 27. - Publication Year :
- 2020
-
Abstract
- Purpose : To evaluate the in vivo efficacy of rose bengal (RB)-mediated photodynamic antimicrobial therapy (PDAT) for treatment of Acanthamoeba castellanii keratitis (AK). Materials and Methods : An animal (rabbit) AK model was successfully achieved via intrastromal inoculation of a suspension of A. castellanii cells and trophozoites. Prior to RB-PDAT (pre-treatment, day-5), the severity of the induced corneal infection was graded numerically for epithelial defects, stromal edema, neovascularity, and stromal opacity/infiltration. The right eyes of rabbits (n = 18) were divided equally into three groups (n = 6/group): control (no treatment); 0.1% RB+518 nm irradiation (5.4 J/cm <superscript>2</superscript> ); and 0.2% RB+518 nm irradiation (5.4 J/cm <superscript>2</superscript> ). On post-treatment day-5, animals were euthanized, after which corneal buttons were excised and submitted for real-time polymerase chain reaction (RT-PCR) analysis. Results : Post-treatment clinical scores of the 0.1 and 0.2% RB groups indicated significant improvement compared to control group scores (pre-treatment clinical scores; 5.17 ± 0.98, 7.50 ± 0.62, and 6.17 ± 0.70 and post-treatment clinical scores; 4.50 ± 0.56, ( p = .043), 3.50 ± 0.99 ( p = .039), 6.83 ± 1.66 ( p = .34), respectively). RT-PCR analysis revealed that the mean cycle threshold (Ct) values were significantly higher in treated-group corneas compared to control-group corneas, with no significant differences between treated-groups (Mean Ct values; 34.33, 34.5, and 29.67 for 0.1 and 0.2% RB, and control groups). There was a statistically significant negative correlation between post-treatment clinical scores and Ct values ( r = -0.474, p -value 0.047). Conclusions : Our results demonstrate that RB-PDAT is effective in decreasing the parasitic load and clinical severity of AK.
- Subjects :
- Acanthamoeba Keratitis diagnosis
Acanthamoeba castellanii drug effects
Acanthamoeba castellanii physiology
Animals
Cornea parasitology
DNA, Protozoan genetics
DNA, Protozoan isolation & purification
Disease Models, Animal
Parasite Load
Rabbits
Real-Time Polymerase Chain Reaction
Acanthamoeba Keratitis drug therapy
Antiprotozoal Agents therapeutic use
Fluorescent Dyes therapeutic use
Photochemotherapy methods
Photosensitizing Agents therapeutic use
Rose Bengal therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1460-2202
- Volume :
- 45
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Current eye research
- Publication Type :
- Academic Journal
- Accession number :
- 32065854
- Full Text :
- https://doi.org/10.1080/02713683.2020.1731830