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Structural characterization of a safflower polysaccharide and its promotion effect on steroid-induced osteonecrosis in vivo.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2020 Apr 01; Vol. 233, pp. 115856. Date of Electronic Publication: 2020 Jan 16. - Publication Year :
- 2020
-
Abstract
- A water-soluble polysaccharide (SPAW) was purified from Safflower and it was identified to be (1→3)-linked β-d-Glucan. The therapeutic effect and underlying mechanism of SPAW on steroid-induced avascular necrosis of the femoral head (SANFH) in a rabbit model was performed here. The abnormal histopathologic changes and apoptosis of femoral head in model group were significantly reverted after SPAW (25, 100 and 200 mg/kg) administration for 60 days, as evidenced by the a decline of empty lacunae rate, the average bone marrow fat cell size and the proportion of apoptotic cells. Furthermore, administration of SPAW significantly decreased the Bax and caspase-3 protein expression, but increased the protein expression of Bcl-2 when compared these in model rabbits. Meanwhile, increased hydroxyproline (HOP) and decreased serum hexosamine (HOM) concentration in rabbit serum were turned to the opposite way. The present study suggested that SPAW may provide an alternative treatment for the treatment of SANFH.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Apoptosis drug effects
Caspase 3 metabolism
Female
Femur Head pathology
Femur Head Necrosis chemically induced
Glucans chemistry
Glucans isolation & purification
Hexosamines metabolism
Horses
Hydroxyproline metabolism
Male
Methylprednisolone Hemisuccinate
Molecular Weight
Proto-Oncogene Proteins c-bcl-2 metabolism
Rabbits
Rats
bcl-2-Associated X Protein metabolism
Carthamus tinctorius chemistry
Femur Head Necrosis drug therapy
Glucans therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 233
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 32059907
- Full Text :
- https://doi.org/10.1016/j.carbpol.2020.115856