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Vulnerability of glutathione-depleted Crassostrea gigas oysters to Vibrio species.

Authors :
Mello DF
Trevisan R
Danielli NM
Dafre AL
Source :
Marine environmental research [Mar Environ Res] 2020 Feb; Vol. 154, pp. 104870. Date of Electronic Publication: 2019 Dec 26.
Publication Year :
2020

Abstract

Glutathione (GSH) is a major cellular antioxidant molecule participating in several biological processes, including immune function. In this study, we investigated the importance of GSH to oysters Crassostrea gigas immune response. Oysters were treated with the GSH-synthesis inhibitor buthionine sulfoximine (BSO), and the function of immune cells and mortality were evaluated after a bacterial challenge with different Vibrio species. BSO caused a moderate decrease (20-40%) in GSH levels in the gills, digestive gland, and hemocytes. As expected, lower GSH decreased survival to peroxide exposure. Hemocyte function was preserved after BSO treatment, however, oysters became more susceptible to challenges with Vibrio anguillarum, V. alginolyticus, or V. harveyi, but not with V. parahaemolyticus and V. vulnificus, indicating a species-specific vulnerability. Our study indicates that in natural habitats or in mariculture farms, disturbances in GSH metabolism may pre-dispose oysters to bacterial infection, decreasing survival.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Details

Language :
English
ISSN :
1879-0291
Volume :
154
Database :
MEDLINE
Journal :
Marine environmental research
Publication Type :
Academic Journal
Accession number :
32056707
Full Text :
https://doi.org/10.1016/j.marenvres.2019.104870