Design and Preparation of Carbon Nitride-Based Amphiphilic Janus N-Doped Carbon/MoS 2 Nanosheets for Interfacial Enzyme Nanoreactor.

Janus amphiphilic particles have gained much attention for their important application value in areas as diverse as interfacial modification, sensors, drug delivery, optics, and actuators. In this work, we prepared Janus amphiphilic nanosheets composed of nitrogen-doped stratiform meso-macroporous carbons (NMC) and molybdenum sulfide (MoS ₂ ) for hydrophilic and hydrophobic sides, respectively. The dicyandiamide and glucose were used as precursors for synthesizing two-dimensional nitrogen-doped meso-macroporous carbons, and the molybdate could be anchored by the functional groups on the surface of carbon layers and then transform into uniformly MoS ₂ to form the Janus amphiphilic layer by layer NMC/MoS ₂ support. Transmission electron microscopy, scanning electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy are used to demonstrate the successful preparation of Janus materials. As the typical interfacial enzyme, Candida rugosa lipase (CRL) immobilized on the Janus amphiphilic NMC/MoS ₂ support brought forth to improvement of its performance because the Janus nanosheets can be easily attached on the oil-aqueous interface for better catalytic activity (interfacial activation of lipases). The obtained immobilized lipase (NMC/MoS ₂ @CRL) exhibited satisfactory lipase loading (193.1 mg protein per g), specific hydrolytic activity (95.76 U g ^-1 ), thermostability (at 55 °C, 84% of the initial activity remained after 210 min), pH flexibility, and recyclability (60% of the initial activity remained after nine runs). In terms of its application, the esterification rate of using NMC/MoS ₂ @CRL (75%) is higher than those of NMC@CRL (20%) and MoS ₂ @CRL (11.8%) in the "oil-water" biphase and CRL as well as NMC/MoS ₂ @CRL in the one-phase. Comparing with the free CRL, NMC@CRL, and MoS ₂ @CRL, the Janus amphiphilic NMC/MoS ₂ served as a carrier that exhibited more optimal performance and practicability.