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A chemically-controlled system for activating RAS GTPases.
- Source :
-
Methods in enzymology [Methods Enzymol] 2020; Vol. 633, pp. 103-117. Date of Electronic Publication: 2019 Nov 22. - Publication Year :
- 2020
-
Abstract
- RAS GTPases are involved in a number of dynamic signaling processes and have been a major focus of research due to the prevalence of activating RAS mutations in cancer. However, despite decades of research, some fundamental aspects of RAS biology are still not well understood. Difficulty in fully defining RAS-driven signaling stems from the overall complexity of downstream pathways and a lack of tools for specifically perturbing RAS function. To better characterize RAS-driven signaling, we recently developed a chemical genetic system for activating endogenous RAS with a small molecule. In this chapter, we describe the use of chemically inducible activator of RAS (CIAR), a single-protein, chemical genetic system that allows the rapid and dose-dependent activation of endogenous RAS. Methods in this chapter also describe the validation of RAS activation with CIAR through the analysis of downstream signaling.<br /> (© 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Signal Transduction
ras Proteins genetics
ras Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1557-7988
- Volume :
- 633
- Database :
- MEDLINE
- Journal :
- Methods in enzymology
- Publication Type :
- Academic Journal
- Accession number :
- 32046841
- Full Text :
- https://doi.org/10.1016/bs.mie.2019.10.033