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Spectroscopic Investigation of DCCH and FTSC as a potential pair for Förster Resonance Energy Transfer in different solvents.
- Source :
-
PloS one [PLoS One] 2020 Feb 11; Vol. 15 (2), pp. e0228543. Date of Electronic Publication: 2020 Feb 11 (Print Publication: 2020). - Publication Year :
- 2020
-
Abstract
- Two molecules, 7-(diethylamino)coumarin-3-carbohydrazide (DCCH) and fluorescein-5-thiosemicarbazide (FTSC) were investigated in different solvents, under varying pH conditions regarding their spectroscopic properties for the usage as a Förster Resonance Energy Transfer (FRET) pair to study the molecular interaction between cellulosic surfaces. All the relevant spectroscopic properties to determine the Förster distance were measured and the performance as a FRET system was checked. From the results, it is clear that the environmental conditions need to be accurately controlled as both, but especially the FTSC dyes are sensitive to changes. For high enough concentrations positive FRET systems were observed in DMF, DMSO, H2O, THF and alkaline DMF. However due to the low quantum yield of the unmodified DCCH throughout the investigated parameter range and the strong environmental dependency of FTSC, both dyes are not preferable for being used in a FRET system for studying interaction between cellulosic surfaces.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Energy Transfer drug effects
Hydrogen-Ion Concentration
Solvents pharmacology
Spectrometry, Fluorescence methods
Spectrophotometry, Ultraviolet methods
Coumarins chemistry
Fluoresceins chemistry
Fluorescence Resonance Energy Transfer methods
Hydrazines chemistry
Solvents chemistry
Spectrum Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 15
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 32045426
- Full Text :
- https://doi.org/10.1371/journal.pone.0228543