Back to Search
Start Over
Oral HPV16 Prevalence in Oral Potentially Malignant Disorders and Oral Cavity Cancers.
- Source :
-
Biomolecules [Biomolecules] 2020 Feb 03; Vol. 10 (2). Date of Electronic Publication: 2020 Feb 03. - Publication Year :
- 2020
-
Abstract
- The role of human papillomavirus type 16 (HPV16) in oral potentially malignant disorders (OPMD) and oral cavity carcinoma (OC) is still under debate. We investigated HPV16 prevalence in unstimulated saliva, oral rinse samples, oral swabs and tumour biopsies collected from OPMD ( n = 83) and OC ( n = 106) patients. HPV16 genotype, viral load, physical status (episomal vs. integrated) and tumour p16INK4a expression were determined. Oral HPV16 prevalence was higher in OC than in OPMD, but this difference was not statistically significant (7.5% (8/106) versus 3.6% (3/83), odds ratio (OR): 2.18, 95% confidence interval (CI): 0.56, 8.48, p = 0.26). There was a significant association ( p < 0.05) between oral HPV16 infection and heavy tobacco consumption. Real-time PCR results indicated that no integration events occurred in either OPMD or OC cases based on the HPV16 E2/E6 ratio. HPV16 positive OPMD and OC patients had similar HPV16 E2 and E6 viral loads. The inter-rater agreement between tumour p16INK4a expression and oral HPV16 infection was considered as fair (k = 0.361) for OC. Our data suggest that the involvement of HPV16 in oral carcinogenesis is limited.<br />Competing Interests: The authors declare no conflict of interest.
- Subjects :
- Aged
Australia epidemiology
Biopsy
DNA, Viral
Female
Genotype
Humans
Male
Middle Aged
Observer Variation
Odds Ratio
Prevalence
Real-Time Polymerase Chain Reaction
Saliva virology
Smoking
Viral Load
Cyclin-Dependent Kinase Inhibitor p16 genetics
Human papillomavirus 16 genetics
Mouth Neoplasms epidemiology
Mouth Neoplasms virology
Papillomavirus Infections epidemiology
Subjects
Details
- Language :
- English
- ISSN :
- 2218-273X
- Volume :
- 10
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biomolecules
- Publication Type :
- Academic Journal
- Accession number :
- 32028653
- Full Text :
- https://doi.org/10.3390/biom10020223