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Scopoletin ameliorates anxiety-like behaviors in complete Freund's adjuvant-induced mouse model.
- Source :
-
Molecular brain [Mol Brain] 2020 Feb 04; Vol. 13 (1), pp. 15. Date of Electronic Publication: 2020 Feb 04. - Publication Year :
- 2020
-
Abstract
- Anxiety disorder is highly prevalent worldwide and represents a chronic and functionally disabling condition, with high levels of psychological stress characterized by cognitive and physiological symptoms. Scopoletin (SP), a main active compound in Angelica dahurica, is traditionally used for the treatment of headache, rhinitis, pain, and other conditions. Here, we evaluated the effects of SP in a mouse model of complete Freund's adjuvant (CFA)-induced chronic inflammation anxiety. SP (2.0, 10.0, 50.0 mg/kg) administration for 2 weeks dose-dependently ameliorated CFA-induced anxiety-like behaviors in the open field test and elevated plus maze test. Moreover, we found that SP treatment inhibited microglia activation and decreased both peripheral and central IL-1β, IL-6, and TNF-α levels in a dose-dependent manner. Additionally, the imbalance in excitatory/inhibitory receptors and neurotransmitters in the basolateral nucleus after CFA injection was also modulated by SP administration. Our findings indicate that the inhibition of the nuclear factor-kappa B and mitogen-activated protein kinase signaling pathways involving anti-inflammatory activities and regulation of the excitatory/inhibitory balance can be attributed to the anxiolytic effects of SP. Moreover, our molecular docking analyses show that SP also has good affinity for gamma-aminobutyric acid (GABA) transaminase and GABA <subscript>A</subscript> receptors. Therefore, these results suggest that SP could be a candidate compound for anxiolytic therapy and for use as a structural base for developing new drugs.
- Subjects :
- 4-Aminobutyrate Transaminase antagonists & inhibitors
Amygdala chemistry
Amygdala drug effects
Animals
Anti-Anxiety Agents pharmacology
Anxiety etiology
Cytokines metabolism
Disease Models, Animal
Drugs, Chinese Herbal pharmacology
Elevated Plus Maze Test
Freund's Adjuvant toxicity
GABA-A Receptor Agonists pharmacology
Inflammation chemically induced
Inflammation psychology
MAP Kinase Signaling System drug effects
Male
Mice
Mice, Inbred C57BL
Microglia drug effects
Models, Molecular
Molecular Docking Simulation
NF-kappa B metabolism
Neurotransmitter Agents metabolism
Open Field Test
Protein Conformation
Receptors, Neurotransmitter metabolism
Scopoletin pharmacology
Angelica chemistry
Anti-Anxiety Agents therapeutic use
Anxiety drug therapy
Drugs, Chinese Herbal therapeutic use
GABA-A Receptor Agonists therapeutic use
Phytotherapy
Scopoletin therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 1756-6606
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Molecular brain
- Publication Type :
- Academic Journal
- Accession number :
- 32019580
- Full Text :
- https://doi.org/10.1186/s13041-020-0560-2