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Influence of sulfonated and diet-derived human milk oligosaccharides on the infant microbiome and immune markers.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2020 Mar 20; Vol. 295 (12), pp. 4035-4048. Date of Electronic Publication: 2020 Feb 03. - Publication Year :
- 2020
-
Abstract
- Human milk oligosaccharides (HMOs) promote the development of the neonatal intestinal, immune, and nervous systems and has recently received considerable attention. Here we investigated how the maternal diet affects HMO biosynthesis and how any diet-induced HMO alterations influence the infant gut microbiome and immunity. Using capillary electrophoresis and MS-based analyses, we extracted and measured HMOs from breast milk samples and then correlated their levels with results from validated 24-h diet recall surveys and breast milk fatty acids. We found that fruit intake and unsaturated fatty acids in breast milk were positively correlated with an increased absolute abundance of numerous HMOs, including 16 sulfonated HMOs we identified here in humans for the first time. The diet-derived monosaccharide 5- N -glycolyl-neuraminic acid (Neu5Gc) was unambiguously detected in all samples. To gain insights into the potential impact of Neu5Gc on the infant microbiome, we used a constrained ordination approach and identified correlations between Neu5Gc levels and Bacteroides spp. in infant stool. However, Neu5Gc was not associated with marked changes in infant immune markers, in contrast with sulfonated HMOs, whose expression correlated with suppression of two major Th2 cytokines, IL-10 and IL-13. The findings of our work highlight the importance of maternal diet for HMO biosynthesis and provide as yet unexplored targets for future studies investigating interactions between HMOs and the intestinal microbiome and immunity in infants.<br />Competing Interests: The authors declare that they have no conflicts of interest with the contents of this article.<br /> (© 2020 Quin et al.)
- Subjects :
- Bacteroides drug effects
Bacteroides isolation & purification
Carbohydrate Sequence
Diet
Electrophoresis, Capillary
Fatty Acids, Unsaturated metabolism
Feces microbiology
Humans
Infant
Infant, Newborn
Interleukin-10 metabolism
Interleukin-13 metabolism
Mass Spectrometry
Neuraminic Acids chemistry
Neuraminic Acids metabolism
Neuraminic Acids pharmacology
Oligosaccharides analysis
Sulfonic Acids metabolism
Th2 Cells cytology
Th2 Cells drug effects
Th2 Cells immunology
Th2 Cells metabolism
Gastrointestinal Microbiome drug effects
Milk, Human metabolism
Oligosaccharides pharmacology
Sulfonic Acids chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 295
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 32014993
- Full Text :
- https://doi.org/10.1074/jbc.RA119.011351