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Regulating Preparation Of Functional Alginate-Chitosan Three-Dimensional Scaffold For Skin Tissue Engineering.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2019 Nov 13; Vol. 14, pp. 8891-8903. Date of Electronic Publication: 2019 Nov 13 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Aim: In this study, we attempted to regulate the preparation of Alg-CS-Flu three-dimensional scaffolds via a facile freeze-drying method combined with amidation.<br />Materials and Methods: Three-dimensional porous flurbiprofen-grafted alginate (Alg)-chitosan (CS) scaffolds were successfully prepared by a facile freeze-drying method combined with amidation for skin tissue engineering applications. Alg-CS composite was first used to load flurbiprofen (Flu), which is a kind of anti-inflammatory non-steroidal molecule. The Flu-loaded Alg/CS composite solution, through freeze-drying and 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide crosslinking to form an Alg-CS-Flu scaffold, exhibited a uniform and porous morphology that was characterized using scanning electron microscopy. The Alg-CS-Flu as-prepared scaffold was also characterized using Fourier-transform infrared spectroscopy, water contact angle, thermal properties, and stress-strain testing.<br />Results: The results reveal that Flu was successfully grafted onto the surfaces of the Alg-CS-Flu scaffold, which showed good hydrophilicity and appropriate mechanical properties. Furthermore, cell viability, cell morphology from cells cultured in vitro, and hematoxylin-eosin staining after the graft was subcutaneously embedded in mice for 7 d demonstrated that the Alg-CS-Flu scaffold had no unfavorable effects on the adhesion and proliferation of fibroblasts, as well as a good anti-inflammatory property.<br />Conclusion: The developed Alg-CS-Flu scaffold is proposed as a promising material or skin tissue engineering application.<br />Competing Interests: The authors report no conflicts of interest in this work.<br /> (© 2019 Zhu et al.)
- Subjects :
- Animals
Cell Proliferation
Cells, Cultured
Fibroblasts cytology
Freeze Drying
Male
Mice
Microscopy, Electron, Scanning
Porosity
Skin cytology
Spectroscopy, Fourier Transform Infrared
Alginates chemistry
Chitosan chemistry
Flurbiprofen chemistry
Skin Transplantation methods
Tissue Engineering methods
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 14
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 32009786
- Full Text :
- https://doi.org/10.2147/IJN.S210329