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Efficiency of Site-Specific Clicked Laccase-Carbon Nanotubes Biocathodes towards O 2 Reduction.
- Source :
-
Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2020 Apr 09; Vol. 26 (21), pp. 4798-4804. Date of Electronic Publication: 2020 Mar 30. - Publication Year :
- 2020
-
Abstract
- A maximization of a direct electron transfer (DET) between redox enzymes and electrodes can be obtained through the oriented immobilization of enzymes onto an electroactive surface. Here, a strategy for obtaining carbon nanotube (CNTs) based electrodes covalently modified with perfectly control-oriented fungal laccases is presented. Modelizations of the laccase-CNT interaction and of electron conduction pathways serve as a guide in choosing grafting positions. Homogeneous populations of alkyne-modified laccases are obtained through the reductive amination of a unique surface-accessible lysine residue selectively engineered near either one or the other of the two copper centers in enzyme variants. Immobilization of the site-specific alkynated enzymes is achieved by copper-catalyzed click reaction on azido-modified CNTs. A highly efficient reduction of O <subscript>2</subscript> at low overpotential and catalytic current densities over -3 mA cm <superscript>-2</superscript> are obtained by minimizing the distance from the electrode surface to the trinuclear cluster.<br /> (© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Details
- Language :
- English
- ISSN :
- 1521-3765
- Volume :
- 26
- Issue :
- 21
- Database :
- MEDLINE
- Journal :
- Chemistry (Weinheim an der Bergstrasse, Germany)
- Publication Type :
- Academic Journal
- Accession number :
- 31999372
- Full Text :
- https://doi.org/10.1002/chem.201905234