Back to Search
Start Over
Label-free electrochemical immunosensor based on biocompatible nanoporous Fe 3 O 4 and biotin-streptavidin system for sensitive detection of zearalenone.
- Source :
-
The Analyst [Analyst] 2020 Feb 17; Vol. 145 (4), pp. 1368-1375. - Publication Year :
- 2020
-
Abstract
- In this study, a sensitive label-free electrochemical immunosensor was designed based on nanoporous Fe3O4 and a biotin-streptavidin system to specifically detect zearalenone (ZEN). Herein, nanoporous Fe3O4 was employed to carry streptavidin to prepare the highly sensitive immunosensor. The application of nanoporous Fe3O4 and the biotin-streptavidin reaction provided large amounts of antibodies on each conjugate, thus amplifying the detected signal. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were conducted to characterize the modification with ZEN. Factors which might influence the properties of the immunosensor, including concentration of nanoporous Fe3O4, pH of the buffer, incubation time and temperature were studied. Under the best conditions, the immunosensor displayed a highly sensitive response toward ZEN, ranging in concentration from 10.0 pg mL-1 to 3.00 ng mL-1 and 3.00 ng mL-1 to 12.0 ng mL-1, with a low detection limit of 3.7 pg mL-1. The results for analysis of human urine samples were satisfactory. Furthermore, this proposed method may find promising applications in the detection of other mycotoxins.
- Subjects :
- Antibodies, Immobilized chemistry
Biosensing Techniques instrumentation
Biotin immunology
Electrochemical Techniques instrumentation
Electrodes
Humans
Immunoassay
Limit of Detection
Reproducibility of Results
Biosensing Techniques methods
Biotin chemistry
Electrochemical Techniques methods
Ferrosoferric Oxide chemistry
Nanopores
Streptavidin chemistry
Zearalenone urine
Subjects
Details
- Language :
- English
- ISSN :
- 1364-5528
- Volume :
- 145
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 31994546
- Full Text :
- https://doi.org/10.1039/c9an02543j