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Combined PGE2 with TNF-α promotes laryngeal carcinoma progression by enhancing GRK2 and TRAF2 interaction.
- Source :
-
Neoplasma [Neoplasma] 2020 Mar; Vol. 67 (2), pp. 354-363. Date of Electronic Publication: 2020 Jan 27. - Publication Year :
- 2020
-
Abstract
- TNF-α has been confirmed to promote tumor growth in LSCC. PGE2 expression in LSCC tissues was significantly higher than in tumor-adjacent tissues. In the present work, we aimed to discover the combined role of TNF-α and PGE2 in LSCC progression and its potential mechanisms. TNF-α and PGE2 were quantified by ELISA. TRAF2, MMP-9 and GRK2 expressions were detected by immunohistochemistry and western blot. UM-SCC-11A cell proliferation was tested by CCK-8, and cell migration and invasion were determined by transwell assay. GRK2/TRAF2 interaction was tested by Co-IP. The results showed that TNF-α, PGE2, TRAF2, MMP-9 and GRK2 expressions were significantly higher in tumor tissues than in tumor-adjacent tissues. Higher expressions of TRAF2, MMP-9 and GRK2 were associated with poorer prognosis of LSCC. Combined TNF-α with PGE2 promoted UM-SCC-11A cell proliferation, migration and invasion. The interactions of TRAF2 and GRK2, as well as MMP-9 expression, were upregulated in response to TNF-α and PGE2 co-stimulation. In conclusion, we found crosstalk between PGE2 and TNF-α signaling pathways, and the interaction between GRK2 and TRAF2 led to the activation of TNF-α-TRAF2-MMP-9 signaling and resulted in the progression of LSCC.
- Subjects :
- Cell Line, Tumor
Cell Proliferation
Humans
Matrix Metalloproteinase 9 metabolism
Signal Transduction
Carcinoma, Squamous Cell pathology
Dinoprostone metabolism
G-Protein-Coupled Receptor Kinase 2 metabolism
Laryngeal Neoplasms pathology
TNF Receptor-Associated Factor 2 metabolism
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0028-2685
- Volume :
- 67
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Neoplasma
- Publication Type :
- Academic Journal
- Accession number :
- 31986892
- Full Text :
- https://doi.org/10.4149/neo_2020_190526N463