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Function and characterization of the promoter region of perilipin 1 (PLIN1): Roles of E2F1, PLAG1, C/EBPβ, and SMAD3 in bovine adipocytes.
- Source :
-
Genomics [Genomics] 2020 May; Vol. 112 (3), pp. 2400-2409. Date of Electronic Publication: 2020 Jan 22. - Publication Year :
- 2020
-
Abstract
- Perilipin 1 (PLIN1) protein, also known as lipid droplet-associated protein, is encoded by the PLIN1 gene and is able to anchor itself to the membranes of lipid droplets. The phosphorylation of PLIN1 is critical for the mobilization of fat in adipose tissue and plays an important role in regulating lipolysis and lipid storage in adipocytes. However, research on the synthesis and lipid metabolism of lipid droplets by PLIN1 in bovine adipocytes is limited. In the present study, we found that bovine PLIN1 was highly expressed in subcutaneous adipose tissue. The highest level of PLIN1 mRNA expression in bovine adipocytes was observed on day 6 of differentiation. Moreover, the cytoplasmic subcellular localization of PLIN1 was observed in bovine preadipocytes. To elucidate the molecular mechanism of bovine PLIN1 transcriptional regulation, we cloned eight fragments containing the 5' regulatory region of the PLIN1 gene. The results showed that the -209/-17 bp region of the bovine PLIN1 gene was the core promoter region. Based on the transcriptional activities of the promoter vector fragments, the luciferase activity of the mutated fragment, the siRNA interference, and the results of the electrophoretic mobility shift assay (EMSA), we identified the binding sites of E2F transcription factor 1 (E2F1), pleiomorphic adenoma gene 1 (PLAG1), CCAAT enhancer binding protein beta (C/EBPβ), and SMAD family member 3 (SMAD3) as the transcriptional activators or repressors of the core promoter region. Further experiments confirmed that the knockdown of the PLIN1 gene affected the ability of these transcription factors to regulate the lipid metabolism in bovine adipocytes. In conclusion, our results reveal a potential mechanism for the transcriptional regulation of PLIN1 in bovine adipocytes.<br />Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest and confirm that the manuscript was approved for publication by all authors.<br /> (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Subjects :
- Adipocytes enzymology
Adipogenesis genetics
Animals
Binding Sites
CCAAT-Enhancer-Binding Protein-beta metabolism
CCAAT-Enhancer-Binding Protein-beta physiology
Cattle metabolism
Cells, Cultured
DNA-Binding Proteins metabolism
DNA-Binding Proteins physiology
E2F1 Transcription Factor metabolism
E2F1 Transcription Factor physiology
Gene Expression Regulation
Lipid Metabolism genetics
Perilipin-1 classification
Perilipin-1 metabolism
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Sequence Analysis, Protein
Smad3 Protein metabolism
Smad3 Protein physiology
Adipocytes metabolism
Cattle genetics
Perilipin-1 genetics
Promoter Regions, Genetic
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8646
- Volume :
- 112
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 31981700
- Full Text :
- https://doi.org/10.1016/j.ygeno.2020.01.012