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Discovery of two novel and adjacent QTLs on chromosome B02 controlling resistance against bacterial wilt in peanut variety Zhonghua 6.
- Source :
-
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2020 Apr; Vol. 133 (4), pp. 1133-1148. Date of Electronic Publication: 2020 Jan 24. - Publication Year :
- 2020
-
Abstract
- Key Message: Two novel and adjacent genomics and candidate genes for bacterial wilt resistance were identified on chromosome B02 in peanut variety Zhonghua 6 using both traditional QTL mapping and QTL-seq methods. Peanut (Arachis hypogaea) is an important oilseed crop worldwide. Utilization of genetic resistance is the most economic and effective approach to control bacterial wilt, one of the most devastating plant diseases, in peanut production. To accelerate the genetic improvement of bacterial wilt resistance (BWR) in peanut breeding programs, quantitative trait locus (QTL) mapping has been conducted for two resistant varieties. In this context, we deployed linkage mapping as well as sequencing-based mapping approach, QTL-seq, to identify genomic regions and candidate genes for BWR in another highly resistant variety Zhonghua 6. The recombination inbred line population (268 progenies) from the cross Xuhua 13 × Zhonghua 6 was used in BWR evaluation across five environments. QTL mapping using both SSR- and SNP-based genetic maps identified a stable QTL (qBWRB02-1) on chromosome B02 with 37.79-78.86% phenotypic variation explained (PVE) across five environments. The QTL-seq facilitated further dissection of qBWRB02-1 into two adjacent genomic regions, qBWRB02-1-1 (2.81-4.24 Mb) and qBWRB02-1-2 (6.54-8.75 Mb). Mapping of newly developed Kompetitive allele-specific PCR (KASP) markers on the genetic map confirmed their stable expressions across five environments. The effects of qBWRB02-1-1 (49.43-68.86% PVE) were much higher than qBWRB02-1-2 (3.96-6.48% PVE) and other previously reported QTLs. Nineteen putative candidate genes affected by 49 non-synonymous SNPs were identified for qBWRB02-1-1, and ten of them were predicted to code for disease resistance proteins. The major and stable QTL qBWRB02-1-1 and validated KASP markers could be deployed in genomics-assisted breeding (GAB) to develop improved peanut varieties with enhanced BWR.
- Subjects :
- Chromosome Mapping
Genetic Association Studies
Genome, Plant
Inbreeding
Microsatellite Repeats genetics
Phenotype
Polymorphism, Single Nucleotide genetics
Reproducibility of Results
Tetraploidy
Arachis genetics
Arachis microbiology
Chromosomes, Plant genetics
Disease Resistance genetics
Plant Diseases genetics
Plant Diseases microbiology
Quantitative Trait Loci genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2242
- Volume :
- 133
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
- Publication Type :
- Academic Journal
- Accession number :
- 31980836
- Full Text :
- https://doi.org/10.1007/s00122-020-03537-9